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EVALUATION OF THE ISOLATION AND DETECTION METHODS

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 اسراء عدنان ابراهيم البغدادي
25/11/2016 10:29:40
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A total of seventy four egg samples were collected from different markets sources, to
investigated for Salmonellaspp. the bacteria was isolated and identified using culturing on
selective media, in addition to, biochemical and serotyping by monovalent antisera.
Multiplex Polymerase Chain Reaction(PCR) detection of theinvA; and DH genes was
used for conformation of the identification of the Salmonella spp.. Twenty eight samples
from seventy four eggs provide positive results with PCR as Salmonella spp. depending
on InvA gene which is the target for the identification at the genus level.

  • وصف الــ Tags لهذا الموضوع
  • Egg shell, invAgene,Multiplex PCR