The aim of this study was to assess the compounds of alkaloids extracts from the leaves of Datura
stramonium, which can be the basis for the synthesis of new antibiotics. In this study, the alkaloid
compounds of D. stramonium have been evaluated. The chemical compositions of the leaves of
ethanolic extract of D. stramonium were investigated using gas chromatography-mass specroscopy.
Gc-MS analysis of alkaloid leaves ethanolic extract of D. stramonium revealed the existence of the Ethyl
iso-allocholate, D-asycarpidan-1-methanol, acetate (ester), 3-(1,5-dimethyl-hexyl)3a,10,10,12btetramethyl1,2,3,3a,
4,6,8,9,10,10a,11,12,12a,12b-tetradec- ahydro -benzo[4,5] cyclohept,2,7-Diphenyl-1,6-
dioxopyridazino [4,5:2,3] pyrrolo[4,5-d] pyridazine, 3,8,8-Trimethoxy-3-piperidyl-2,2-benaphthalene-
1,1,4,4-tetrone, [5?] Pregnane3, 20?-diol,14?,18?-[4-methyl,3-oxo-[1-oxa-4-azabutane-1,4-diyl], diacetate,
1-monolinoleoylglycerol trimethylsilyl ether and 17-[1,5-dimethylhexyl]-10,13-dimethyl-
3sstyrylhexadecahydrocyclopenta[a]phenathren-2-one. Alkaloids extract from leaves of D. stramonium
were assayed for in vitro antibacterial activity against Escherichia coli, Proteus mirabilis,
Staphylococcus aureus, Pseudomonas aerogenosa and Klebsiella pneumonia by using the diffusion
method in agar. The zone of inhibition was compared with different standard antibiotics. The diameters
of inhibition zones ranged from 0.8 to 2.01 mm for all treatments.