Phytochemicals are chemical compounds formed during the plants normal metabolic processes. These
chemicals are often referred to as secondary metabolites. The objective of this research was to
determine the chemical composition of leaves extract from methanol. The phytochemical compound
screened by gas chromatography-mass spectrometry (GC-MS) method. Fifteen bioactive phytochemical
compounds were identified in the methanolic extract of Urtica dioica. The identification of
phytochemical compounds is based on the peak area, retention time molecular weight, molecular
formula, mass spectrometry (MS) fragment-ions and pharmacological actions. GC-MS analysis of U.
dioica revealed the existence of the Oxime- methoxy-phenyl, 2, 6,-Nonadienal, 3, 7-dimethyl, 1, 2, 3-
Butanetriol, Silane, triethyl(2-phenylethoxy), Benzofuran, 2,3,-dihydro, 2,5,5,8a-Tetramethyl-1,2,3,5,6,7,8,
8a-octahydronaphthalen-1-ol, 2H-Indeno[1,2-b]furan-2-one, 3,3a, 4,5,6,7,8, 8b-octahydro-8,8-dimet, 1-
Dodecanamine, N, N-dimethyl, 2(3H)-Naphthalenone, 4, 4a,5,6,7,8-hexahydro-1-methoxy, D-Fructose,
diethyl mercaptal, pentaacetate, [1,1-Bicyclopropyl-2-octanoic acid 2hexyl-methyl ester, Estra-1,3,5(10)-
trien-17B-ol, Cyclopropaneoctanoic acid, 2-[2-pentylcycloproyl)methyl]-methyl, 1-Hydroxy-2-(2,3,4,6-
tetra-O-acetyl-beta-d-glucopyranosyl)-9H-xanthe and Ethyl iso- allochlate. The FTIR analysis of U. dioica
leaves proved the presence of aromatic rings, alkenes, aliphatic fluoro, alcohols, ethers, carboxlic
acids, esters, nitro compounds, hydrogen bonded alcohols and phenols. It contain chemical
constitutions which may be useful for various herbal formulation as anti-inflammatory, analgesic,
antipyretic, cardiac tonic and antiasthamatic.