ABSTRACT
Low level laser therapy (LLLT) empirically used in the treatment of various medical conditions stimulating
certain intracellular biological activities resulting in acceleration of wound healing due to various biostimulatory
effects. It has been widely tested on cell culture studies; the mechanism of action is complex, in general it involves
absorption of light on subcellular level particularly the electron chain system in the mitochondria resulting in the
biostimulatory effect.
The chronic inflammatory process have been shown to be reduced in periodontal tissue by LLLT with
subsequent release of metalloproteinase’s and collagenases resulting in improvement in tissue healing and wound
remodeling. It has been also shown to cause smooth muscle relaxation and subsequent vasodilatation. Several studies
in vitro have shown that LLLT at certain wavelengths may stimulate fibroblast proliferation. Furthermore; (He-Ne)
LLLT has been shown to have stimulatory effect on cultured osteoblast resulting in enhancement of proliferation,
differentiation and calcification. Other empirical uses of laser therapy include analgesia, antimicrobial activity, laser
curettage and relieving trigeminal neuralgia.
Objectives and Results: This study was designed to measure two of the coagulation factors of whole blood samples
obtained from 25 healthy male and female adult volunteers before and after LLLT using three different wavelengths
(650, 532 and 405 nm), namely factor VIII and von-Willebrand factor; both of these factors are available within
platelets granules. The mean concentration of VIII and vWF in all samples were 1.15 and 4.46 ng/ml respectively.
The mean concentration and the percent change in individual samples of VIII and vWF after LLLT are listed
respectively as follows: 650 nm (0.84, 6.23 and 4.42, 1.48), 532 nm (0.97, - 8.23 and 4.17, -4.24), and 405 nm (0.91,
5.81 and 4.33, -1.42). Paired T-test showed no significant change on VIII including the concentration and percent.
The percent change was in VIII concentration was in general towards a negative value for both 650 and 532 nm