Nineteen isolates of Gram negative bacteria were isolated and identified out of 43 urine samples of diabetic women. The isolates showed a high resistance to antibiotic ampicillin, amoxicillin and carpinicillin, and intermediate resistance to gentamycin, kanamycin, chloramphnicol and naldixic acid, while show low resistance to ciproflaxin, and doxycyclin. Also they showed no ability to produce bacteriocin except only one isolate of Klebsiella pneumonia which had the ability to produce bacteriocin. The plasmid profile of the isolates showed that 10 isolates have mega plasmid but only one isolate have small plasmid.