ABSTRACT:
The most prevalent complication of gallstone is chronic cholecystitis. The aim of the present study was to determine the responsible microorganisms association in gallbladder inflammation in patients undertook cholecystectomy and determine the relationship of serum interlukin-12 concentration and some hematological parameter with gall bladder infaction.

Cholecystectomy was achieved in 107 patients. Collected samples transferred to laboratory inappropriate media, then cultured on selective media to isolate the possible causative bacteria.the levels of interleukin-12 , PCV, WBC,HB were determined.
bacterial growth was shown in 94.4% of all cultured samples , of which 87.6% gram negative bacteria (E.coli, pseudomonas,Enterobacter,Klebsiella,salmonella, shigella, Acintobacter, Citrobacter,Proteus).and 12.4% grame positive bacteria(Enterococcus feacales,Staphylococcus.areuse,Staphylococcus.epidermidis,Bacillus,Streptococcus.pneumonia, and Streptococcus viridians ).
the resulte explain increase in levels of interleukin-12 and variation in hematological parameters compaired with control. The study also revealed three types of stones depending upon their colouer(36 cholesterol, 26 mixed, 34 pigment) . High percentage of bacteria was isolated from pigment stone,where as cholesterol stone show no bacterial growth.