Isolation and identification of Campylobacter jejuni from diarrhea of children.
Lumma J. H. Witwt Azhar – AL- Thahab Frial G.Abd
Babylon University Babylon University College of science
College of dentistry
Abstract:
One handred twenty five stool samples were collected from children with diarrheal disease in age between (1-5) years old who attending the Babylon hospital for maternity and children from the period November 2009 to May 2010. C.jejuni was isolated from 12 diarrheal samples with percentage 40% using two methods. First the using selective media (Skirrow’s Media) and second by using filter paper with pore size (0.45?m). Biochemical, physiological and antibiotics sensitivity tests were conducted for 12 bacterial isolates. All of these isolates were sensitive to nalidixic acid and resistance to cephalothine in percentage 100%. Local specific antibodies was detected using tube agglutination test for globulins isolated from stool samples which was positive for 30 samples with percentage 54.5%. Rapid latex agglutination test was positive also for these samples.