Abstract
This study was carried out for the isolation and identification of Pseudomonas aeruginosa from 260
patients who were suffering from: burns, chronic otitis media and urinary tract infections (UTI).
Detection of the isolates susceptibility for 23 antibiotics was also studied. Moreover, chemical
substance like ethylene diamine tetra acetate (EDTA) was added to some antibiotics in an attempt to
enhance their effects.
The total number of P.aeruginosa isolates accounted for 30 of 260 samples, the distribution of
those isolates were: 10 from 32 burn samples,10 from 58 otitis samples and 10 from 170 UTI samples.
The results showed high degree of resistance for most antibiotics being used in this study. Also
results were indicated for extended-spectrum beta-lactamases production by this bacteria by using
EDTA as indicator and the positive results were observed in 9(30%) isolates with ampicillin and
12(40%) isolates with cefixime.