Polycystic ovary syndrome (PCOS) is one of the most common endocrine dysfunctions in women of reproductive age .it isrefers to a heterogeneous group of gynecologic disorders with variable degrees of ovarian and adrenal hyperandrogenism. Tumor necrosis factor-? are related with gynecological pathologies including preeclampsia, endometriosis and PCOS. Insulin resistance and TNF-? was measured from fasting glucose, TNF-? gene polymorphisim estimation using PRC-RFLPin a group of 50 PCOS females and 50 age compare with healthy controls. The age in both study groups no significant difference for patients (22.44 ± 3.20) and controls (23.34 ± 2.95). All the PCOS had elevated body mass index (27.70 ± 5.07), waist to hip ratio (0.85 ± 0.04), fasting insulin (10.76 ± 2.86), homeostatic model assessment (HOMA) score (3.18 ± 1.14) and serum TNF-? (23.19 ± 3.95), when compared with controls. There was a significant (r=0.244, P=0.01), (r=0.293, P=0.003) positive correlation of IR with BMI and waist to hip ratio, respectively.
Also, a significantly (r=0.543, P<0.001) positivecorrelation were found between TNF-? and insulin resistance. Genotype
distribution for the C-850T polymorphism was observed with the frequency of the variant T allele being 16% in the
PCOS group and 6% in the control group (p>0.11). In conclusion, present examinations suggested that the TNF-?
might contribute to the pathogenesis of PCOS, being the basis of increase body weight which lead to development of
insulin resistance. While the incidence of PCOS independent of a polymorphism of the TNF-? C850T (rs 1799724) in
population studied.
Key words : Polycystic ovary syndrome, Tumor Necrosis Factor Alpha, Insulin resistance, Polymorphism of TNF-? C850T
(rs1799724).