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Isolation of Bacteria from PatientsWith Blepharitis and Investigation of Beta-Lactamase Production

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 نيران كاظم فرهود الربيعي
17/02/2019 06:53:10
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In this study 31 eyelids swabs were obtained from patients suffering from blephatitis of different age groups and
both sexes in Hilla province.
Bacterial infections were detected in 26 (83.9%) samples and no growth resulted in 5 (16.1%) samples . It was found
that Staphylococcus aureus was the commonest bacteria ( 15 isolates ), followed by Staphylococcus epidermidis ( 7
isolates ) and only ( 4 isolates ) of Streptococcus pneumoniae .
The rapid iodometric method has been employed to investigate the production of beta-lactamase enzyme by
common bacteria Staphylococci (Staphylococcus aureus and Staphylococcus epidermidis ) which isolated from
eyelid s margin of patients with blepharitis . It was found that (13) isolates of Staphylococcus aureus and (5) isolates
of Staphylococcus epidermidis have revealed positive results.
A number of common Beta-lactam antibiotics such as Penicillin, Ampiclox , Amoxicillin , Cephalexin and
Cefazolin have also been used to investigate the ability of Staphylococcus aureus and Staphylococcus epidermidis to
produce ?-lactamase enzyme . The results showed that Staphylococci (Staphylococcus aureus and
Staphylococcus epidermidis ) were 100% resistant to Penicillin , Cephalexin and Cefazolin , other antibiotics
Ampiclox and Amoxicillin showed remarkable variations in respect of resistance ratio .

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  • In this study 31 eyelids swabs were obtained from patients suffering from blephatitis of different age groups and both sexes in Hilla province.