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Isolation and Characterization of Bacteria from Patients with Conjunctivitis in Hilla Province

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 نيران كاظم فرهود الربيعي
17/02/2019 06:49:55
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This work therefore, aims to study the isolation and identification of common bacterial
pathogens associated with conjunctivitis and examine some virulence factors such as (Capsule,
Hemolysin, Siderophores, Extracellular Proteases and Adherence Factor and Show the effect of some
antibiotics on bacterial isolates.
In our study, 120 conjunctival swabs were subjected to isolate and identify the bacterial strains
associated with conjunctivitis. These swabs were obtained from patients suffering from conjunctival
inflammation of both sexes who were attended to the Hilla Teaching Hospital/ Ophthalmic Unit in
Hilla province.
Bacterial infections were detected in 82 (68.3%) samples and no growth resulting in 38
(31.7%) samples. It was indicated that Haemophilus influenzae was the first colonizer of the
conjunctival sac (49 isolates) among the children and adults, followed by Staphylococcus aureus (19
isolates), Streptococcus pneumoniae (7 isolates) and a few number (4 isolates) of Neisseria
gonorrhoeae on the first week of life were isolated, an act, which confirmed the predominance of
Gram-negative bacteria early in life.
Also (3 isolates) of Klebsiella pneumoniae were isolated from children below one year old and
from adults. Some virulence factors of these bacterial isolates were investigated. Also, invitro effect of
some antibiotics on these bacterial isolates was studied, and the results showed that Ciprofloxacin and
Chloramphenicol were the most sensitive among the other drugs; hence they should be the first choice
for the treatment of bacterial conjunctivitis.

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  • This work therefore, aims to study the isolation and identification of common bacterial pathogens associated with conjunctivitis and examine some virulence factors such as (Capsule, Hemolysin, Siderophores, Extracellular Proteases and Adherence Factor and Show the effect of some antibiotics on bacterial isolates.