Objective:-
Detection of interleukin-33 gene polymorphism from patients and control DNA.
Materials and Methods:
Blood samples were collected from 120 patients with gyneacological and breast cancer, and 79 healthy women were control group . rs1929992 (in IL-33 gene) were determined using PCR-RFLP.
Results:-
The statistical analysis and data presentation in the present study was based on inclusion of 120 women with cancer (uterine, breast, ovarian and cervical) serving as a study group and 79 apparently healthy women serving as a control group.
The prevalence rate of homozygous wild genotype (A / A), related to IL-33, was 74.7 % (59 out of 79) in control group and 49.2 % (59 out of 120) in study group. In addition, the prevalence rate of heterozygous wild and recessive genotype (A / G), related to IL-33, was 15.2 % (12 out of 79) in control group and 32.5 % (39 out of 120) in study group. Moreover, the prevalence rate of homozygous recessive genotype (G / G), related to IL-33, was 10.1 % (8 out of 79) in control group and 18.3 % (22 out of 120) in study group.
The difference in these rates was highly significant (P = 0.002); it was obvious that women with cancer expressed the homozygous recessive allele (G / G) of IL-33 in higher rate than control women, 18.3 % versus 10.1 %; it was also obvious that women with cancer expressed the homozygous wild allele (A / A) of IL-33 in lower rate than control women, 49.2 % versus 74.7 %.
Wild IL-33 allele was significantly less frequent in study group than in control group, 65.4 % versus 82.3 % (P < 0.001), whereas, recessive IL-12 allele (G) was significantly more frequent in study group than in control group, 34.6 % versus 17.7 % (P < 0.001).

Women with cancer expressed the homozygous wild allele (A\A) of IL-33 in lower rate than control women.
The wild IL-33(A) allele may play a protective role against cancer.
IL-33 was significantly associated with cancer, with 2.45 fold risk, and this allele was most frequently associated with cervical cancer.
These facts suggest that the interaction between genetic factors, represented by Il-33 G allele, and environmental factors, leads to initiation, propagation and establishment of malignant lesions.


وصف الــ Tags لهذا الموضوع   Gyneacological cancer ,Breast cancer, Interleukin-33, Single nucleotide –polymorphism.