Detection of Human Papilloma Virus DNA among Patients with Gyneacological and Breast Cancer

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ميس هادي جبر
01/04/2019 05:21:39

Objective: Detection of viral DNA from patients and sequencing analysis and NCBI data base regestration of some HPV positive samples. DNA sequencing method was performed for confirmative genetic identification and phylogenetic tree analysis of HPV 16 genotype. The positive the PCR product of HPV L1 gene were sent to Macrogen Company in Korea for performed the DNA sequencing by AB DNA sequencing. The statistical analysis and data presentation in the present study was based on inclusion of 120 women with cancer (uterine, breast, ovarian and cervical) serving as a study group and 79 apparently healthy women serving as a control group. Overall 27 women with cancer out of 120 showed positive results, after PCR examination, accounting for 77.5%. Out of those 27 women, 24 had HPV-16 serotype. Comparison of mean age between those women with positive HPV infection and those women who are negative for HPV revealed no significant difference (P = 0.229) and mean age was 56.11 ± 9.31 years versus 53.54 ± 9.85 years in both categories, respectively. We were able to isolate HPV-16 strain and after DNA sequencing we were able to both sketch the phylogenetic tree and register the strains in the gene bank.

وصف الــ Tags لهذا الموضوع   Breast cancer, Interleukin-12, Interleukin-33, Single nucleotide-polymorphism, Tumor stages Gyneacological cancer, Breast cancer, HPV infection, HPV-16, SNP.