Detection of Rota virus by rapid test in comparison with enzyme linked immunoassay in acutediarrhea children in Babylon province



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أيام محمد صالح علي العامود
04/04/2019 07:01:54

Abstract
Globally, human rotavirus is considered as a major etiologic agent of acquired diarrhea which infect young kids as well as infants. For the purpose of efficient treatment, early diagnosis is of high importance.Group-A Rotavirus are in responsible forserious watery diarrhea in young kids. Rapid analysis of the Rotavirus associated diarrhea can prevent inappropriate supervision of antibiotics and help in stopping the pass on of multi- drug resistance. Rotavirus antigen is discovered by ELISA and with one step rapid antigen test showed an occurrence of 23% using ELISA and 22.5% using Rapid test respectively. Fast showed a tenderness of 96.97% and specificity of 100% in comparison to ELISA. The Rotavirus infection was maximum in age group of one years to two years (60.86%) in male and in addition tofemale (39.13%). Throughout the winter, the infections was at its highest levels, also ithas been appeared with triad of diarrhea, fever and vomiting. Severe dehydration and watery diarrhea has been the main cases of this infection.
K ey w o rd s: ELISA, Group A Rotavirus, Immunochromatography, Watery diarrhea
Introduction
Extreme diarrheal disease is a significant public health problem leading to notable rate of death and morbidity in advanced and emergingnations such as India. Globally, the infection of Rota virus is considered as the third most widespread reason of severe diarrhea in young kids[1].The vast majority of kids experiencedthe infection of Rota virus in the course of time when they are five years of age. Approximately that Rota Virus infection annually result in 111 million episodes of gastroenteritis which requirescare at home, five million clinicvisit, 1 millionhospital admission and about600mortality rates in kids less than five years oldthroughout the world[2]. Presently there are seven Rota virus groups seen to infect the humans, among them the most dominant is group A [3]. WHO estimated that diarrhea is in charge of 18% deaths among children less than five years of time [4]. Every year, there are some seasonal peaks of diarrhea in Bangladesh and ICDDR,B alone has to treat more than 100,000 patients a year[5]. The data on clinical characteristics and treatment outcome of rotavirus diarrhea are likely to be helpful for the medical professionals and health care providers working at the grass root levels who have the tendency to refer any acute watery diarrhea with or without vomiting to other secondary/tertiary level hospital for “better management[6]. This study was intended to explore the clinical severity, nature of disease and to observe the treatment outcome of both Rota and non?rotavirus diarrhea in children of Bangladeshthe justification of such a huge
referral of diarrhea cases to higher centers. [7].One of the main causes of illness and mortality in Iraqi kids is Diarrhea. Nevertheless, someresearchers in Iraqreportedvariousdegrees of infectivity with Rotavirus in the governorates of Iraq. Rotavirus is accountable 30 percent of acute diarrhea in hospitalized kids in Baghdad, 18.5 percent in Tikrit, 24 percent in Basra, 37 percent in Erbil[8-10]. So,the presentedresearchhas beenconducted for estimating the spread of Rotavirus in the governorate of Babylon through utilizing 3 commercial diagnostic kits, followed by a study to detect the rotavirus genotyping circulating between populations through the use of RT-PCR molecular diagnostic kit[11].

وصف الــ Tags لهذا الموضوع   Globally, human rotavirus is considered as a major etiologic agent of acquired diarrhea which infect young kids as well as infants.