Isolation from diseased plants:
the most common method however for isolating pathogens from
infected plant parts involve
2- Direct study:
specimens
that are studied directly include mushrooms and the larger fungi, which
represent the final stage in the reproductive cycle. Fungi such as rusts,
smuts, mildews and other obligate parasites that can not be cultured, are also
studied in this way. A microscopic preparation can be made directly from the
material