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Reticulocyte Count

الكلية كلية طب الاسنان     القسم  العلوم الاساسية     المرحلة 2
أستاذ المادة وسن نجم عبد السادة الربيعي       22/02/2019 23:01:10
RETICULOCYTE COUNT
Reticulocytes have the same biconcave discoid shape as mature red cells erythrocytes, they are still contain basophilic ribonucleic protein but no nucleus (fif.1).
The reticulocytes pass into the blood capillaries. The remaining basophilic material in the reticulocyte normally disappears within one to two days and become mature erythrocyte.Because of the short life of the reticulocytes, their concentration among all the red cells of the blood is normally slightly less than 1% (tab.*).







Staining Solution:
Brilliant cresyl blue 1.0 gm
Sodium carbonate 400 gm
Normal saline 100 ml

Dissolve the dye in normal saline then add sodium carbonate. Mix and filter the stain and keep at room temperature.


Procedure:
1. Put 3-4 drops of staining solution in a small test tube.
2. Add equal amount of blood. If patient is anaemic add more blood. If patient is polycythemic add less blood.
3. Mix and keep in incubator at 37 0C for 15-20 minutes. Gently mix the solution.
4. Prepare thin smear using 1 small drop blood mixture, let it to dry.
5. Under oil immersion, count all red cells in each field where the cells do not overlap, including of reticulocytes.
6. Count 1000 red cells in the field. Then calculate the percentage of reticulocytes.

Calculation:
The percentage of reticulocytes is


Reticulocyte % =
10

The absolute reticulocyte count may be calculated by the following formula:


Absolute reticulocyte =
count 100



NOTE:
The reticulocyte count is most often expressed as a percentage of total red cells.


المادة المعروضة اعلاه هي مدخل الى المحاضرة المرفوعة بواسطة استاذ(ة) المادة . وقد تبدو لك غير متكاملة . حيث يضع استاذ المادة في بعض الاحيان فقط الجزء الاول من المحاضرة من اجل الاطلاع على ما ستقوم بتحميله لاحقا . في نظام التعليم الالكتروني نوفر هذه الخدمة لكي نبقيك على اطلاع حول محتوى الملف الذي ستقوم بتحميله .