انت هنا الان : شبكة جامعة بابل > موقع الكلية > نظام التعليم الالكتروني > مشاهدة المحاضرة

Haemoglobin Estimation

الكلية كلية طب الاسنان     القسم  العلوم الاساسية     المرحلة 2
أستاذ المادة وسن نجم عبد السادة الربيعي       21/02/2019 19:12:40
HAEMOGLOBIN ESTIMATION

Haemoglobin: is the most important pigment of the blood imparting red colour to it. The main function of Hb is oxygen carrying from lungs to the body tissues and the Co2 transport in the blood.
Haemoglobin is a large complex molecule with a quarternary structure of four globular protein chains, each of is wrapped around an iron-containing heme group (fig.1).



































The object of estimating haemoglobin (Hb) is to determine the oxygen – carrying capacity of the blood.
The results assist in :
a. detecting diseases which cause a deficiency of excess of Hb .
b. in studying changes in Hb concentration before or after operations and blood transfusion.
Generally, Hb estimation rely on a coparism of colours. Depend upon matching the colour produced by the test sample with the colour produced by a standard sample of know Hb concentration.




Some of the common methods used in Hb estimation are:
1. Use of haemoglobinometer
- Shali’s or (acid haematin) method
- Haldane’s method
2. Use of colorimeter
- Oxy-Hb method
- Cyanomethaemoglobin method
3. Estimation of iron inHb





Shali’s or (Acid Haematin) Method

Principle:
Hb in the red blood cells s converted into acid haematin by the action of HCL. The brown colour developed is matched against standard brown-tinted glass in the comparator by direct vision.


Apparatus and reagents:
a. Haemoglobinometer (Shali’s):the box contains:
1. Comparator
2. Haemoglobin tube (Shali Adams tube)
3. Haemoglobin pipette
4. A thin glass-rod strirer
b. 10N/ HCL (0.1N HCL) solution



Procedure:
1. Fill the graduated (Shali Adams) tube to the 20 mark (on % scale) with 0.1 HCL (fig.2B).
2. Draw blood by using haemoglobin pipette to the 0.02 ml (fig.2A).
3. Wipe the tip of the pipette with cotton so that no blood is left to stick to its outside.
4. Expel the blood into the Shali tube containing the HCL solution. Suck a small amount of an acid into the pipette and expel it again into the tube, repeat this twice.
5. Mix the content quickly but gently with glass-rod and leave for10 min.
6. Add distilled water drop by drop, mixing between each addition until the colour matches with standard.
7. Read the amount of solution in the graduated tube. The calibrations give the Hb concentration.


-Normal values are shown in tab.*


المادة المعروضة اعلاه هي مدخل الى المحاضرة المرفوعة بواسطة استاذ(ة) المادة . وقد تبدو لك غير متكاملة . حيث يضع استاذ المادة في بعض الاحيان فقط الجزء الاول من المحاضرة من اجل الاطلاع على ما ستقوم بتحميله لاحقا . في نظام التعليم الالكتروني نوفر هذه الخدمة لكي نبقيك على اطلاع حول محتوى الملف الذي ستقوم بتحميله .