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الكلية كلية الصيدلة
القسم فرع العقاقير والنباتات الطبية
المرحلة 2
أستاذ المادة سعد علي احسان الاعرجي
5/25/2011 9:49:16 PM
Lecture ( 11 B )
6.Gel chromatography.
The separation occurs on the basis of adsorption , partition &on the effective size of solutes present in the solution for the separation purpose , the stationary phases used are cross- linked polymers which give on open network with large number of pores of fairly uniform size. During the flow of mobile phase through such stationary phase ,many large size molecule excluded & travel along with mobile phase , the low molecular size enter freely in to different pores . During the elution the largest molecules in the mobile phase elute first ,followed by molecules with decreasing order in size .
The types of gel used as stationary phase are either soft gel or semi-rigid or rigid gel, soft gel like agarose ,dextran &poly acryamide .The semi-rigid or rigid gel are alkylated dextran , controlled –porosity glass beads. Soft gel are used with aqueous mobile phases (the process called gel filtration ) ,the other gel are used non-aqueous mobile phase like acetone , chloroform ….etc (the process called as gel permeation ).
7.Affinity chromatography.
This technique used for separation of protein , m-RNA ,peptide ,enzymes antigens &antibodies. The adsorbent used is one of the biological substance called receptor a specific affinity for other substances i:e the two substance are biologically interacting . The adsorbent is attached to a porous stationary phase &placed in column , when mixture containing the other complement of the adsorbent is passed through the stationary phase i:e select separation during the elution ,the complementary part adsorbed is collected in pure form by dissociating the interacting pair with help of changing the ionic strength or PH of the column buffer.
Electrophoresis
For the electrophoretic separations a filter paper strip is impregnated with a solution of an electrolyte (usually buffer solution )& supported in the center , its two ends are dipped in to solution in which electrodes are immersed . A spot of the material to be fractionated is placed on the paper .According to the charge on the ions of solute mixture , the solutes will move towards either the anode or cathode ,paper can be replaced by thin layers of gel (gel electrophoresis) ,also there is capillary electrophoresis give efficiencies separation &wih detector systems such as laser-induced fluorescence.
Dr. Ghada Alhusaini
المادة المعروضة اعلاه هي مدخل الى المحاضرة المرفوعة بواسطة استاذ(ة) المادة . وقد تبدو لك غير متكاملة . حيث يضع استاذ المادة في بعض الاحيان فقط الجزء الاول من المحاضرة من اجل الاطلاع على ما ستقوم بتحميله لاحقا . في نظام التعليم الالكتروني نوفر هذه الخدمة لكي نبقيك على اطلاع حول محتوى الملف الذي ستقوم بتحميله .
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