انت هنا الان : شبكة جامعة بابل > موقع الكلية > نظام التعليم الالكتروني > مشاهدة المحاضرة
الكلية كلية الطب
القسم الكيمياء الحياتية
المرحلة 2
أستاذ المادة عبد السميع حسن حمود الطائي
5/7/2011 9:29:34 PM
Subject: Determination of Blood Glucose Time: Two Hours
Second Class No. of students: 30
Lecturer Abdulsamie Hassan Alta ee
Introduction:
Glucose is the most important carbohydrate fuel in the body. In the fed state, the majority of circulating glucose comes from the diet; in the fasting state, gluconeogenesis and glycogenolysis maintain glucose concentrations. Very little glucose is found in the diet as glucose; most is found in more complex carbohydrates that are broken down to monosaccharides though the digestive process. About half of the total carbohydrates in the diet are in the form of polysaccharides and the remainder as simpler sugars. About two-thirds of the sugar in the diet is sucrose, which is a disaccharide of glucose and fructose.
Ordinarily the concentration of glucose in the blood is maintained at a relatively stable concentration from 80 to 120 mg/dl. The strong reducing properties of glucose made it relatively easy to measure and thus the clinical estimation of circulating glucose was one of the earliest tests available to the clinician. The recent introduction of microglucose oxidase technology has now made it possible for the patient to measure his or her own blood glucose concentration and undoubtedly makes the estimation of blood glucose the most widely used test of blood chemistry. An understanding of the methods of blood glucose measurement will help the clinician to interpret values and avoid the pitfalls of inaccurate testing.
Learning Objectives:
At the end of biochemistry laboratory, the students should be able to:
1. Describe the importance of glucose metabolism.
2. Describe the normal values of blood glucose.
3. Describe the hyperglycemia and diseases associated with.
4. Describe the hypoglycemia and diseases associated with.
5.
Contents:
1. Summary about glucose metabolism.
2. Role of insulin in glucose metabolism.
3. Principle of method
4. Procedure
5. Calculation
6. Reference range
7. Clinical significant
8. References
Material and Instruments:
1. Blood glucose kit
2. Blood sample
3. Centrifuge
4. Spectrophotometer
Laboratory Subject:
1. Prelaboratory talk about glucose metabolism and the role of insulin in glucose metabolism as well as, talk about hyperglycemia, hypoglycemia and diseases associated with them.
2. Blood withdrawing.
3. Preparation of blood plasma.
4. Determination of blood glucose.
5. Writing a laboratory report.
6. Solve a quiz.
Student Work
The students should able to:
1. Participates actively in the laboratory.
2. Withdraws blood sample
3. Prepares blood plasma.
4. Follows the procedure to determine the blood glucose.
5. Discuses the result obtained and suggest the diagnosis.
6. Writes a laboratory report.
References
1. Mufeed J. Ewadh and Fadhil J. Altoma, Maniual of practical clinical biochemistry for 2nd year students,2000, College of Medicine ,Universty of Babylon.
2. Murray R.K., Granner D.K., Mayes P.A. and Rodwell V.W., Harper’s Biochemistry, 24th ed., Stamford, Appleton and Lange (2005).
3. Luxton R., Clinical Biochemistry, 1st ed., Oxford, Butterworth-Heinemann (1999).
4. Bishop M.L., Duben-Engelkirk, and Fody E.P., Clinical Chemistry, 4th ed. Philadelphia, Lippincott Williams & Wilkins (2000).
5. Lehninger A.L., Principles of Biochemistry, 3rd ed., New York, Worth publishers (2005).
6. Burtis C.A. and Ashwood E.R., Tietz Textbook of Clinical Chemistry, 3rd ed., Philadelphia, W.B.Saunders Co. (1999).
7. Varley H., Gowenlock A.H. and Bell M., Practical Clinical Biochemistry Vol.1, 5th ed., London, William Heinemann Medical Book Ltd. (1991).
8. Marshall W.J., Clinical chemistry, 2nd ed., London, Gower Medical Publishing (1992).
9. Mayne P.D., Clinical Chemistry in Diagnosis and Treatment, 6th ed., London, Arnold a member of the Hodder headline Group plc. (1998).
المادة المعروضة اعلاه هي مدخل الى المحاضرة المرفوعة بواسطة استاذ(ة) المادة . وقد تبدو لك غير متكاملة . حيث يضع استاذ المادة في بعض الاحيان فقط الجزء الاول من المحاضرة من اجل الاطلاع على ما ستقوم بتحميله لاحقا . في نظام التعليم الالكتروني نوفر هذه الخدمة لكي نبقيك على اطلاع حول محتوى الملف الذي ستقوم بتحميله .
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