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Fungi

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الكلية كلية التمريض     القسم قسم العلوم الطبية الاساسية     المرحلة 2
أستاذ المادة ميس هادي جبر       20/02/2019 08:38:24
Fungi lab:1
Fungi grow at comparatively slow rates , are requiring several days to weeks to form macroscopically visible colonies . Usually, the growth will spread over the entire culture plate .
Fungi produce spores on brightly colored aerial hyphae .
Most fungi grow best at room temperature 25C rather than at 35C . The basic medium for the culture of many molds (multicellular, filamentous fungi ) is sabouraud dextrose agar ,the high sugar concentration and low pH (5.6 ) of this medium make it unsuitable for the growth of most bacteria, thus guarding against contamination -fungi grow well at pH 5.6 mold colonies may be examined directly in culture with a dissecting microscope .
However, it is better to teach away apportion of the growth and place it on a slide with a drop of water or stain ( a wet - mount) . An alternative method is a slide culture . the latter is more advantageous because one is able to study the growing mold and identify specific structures without disturbing the culture , in this way the reproductive structure can The macroscpic aggregation ( colony ) of mold cells is called thallus, athallus is composed of mass of strands called a mycelium, each strand is a hypha.
vegetative hyphae grow on the surface of culture media, they form aerial hyphae, called reproductive hyphae , that bear asexual reproductive spores or conidia .
The hyphae that grow below the surface of culture media are called rhizoidal hyphae.
The hyphal strand of some molds may be separated by across wall called a septum. hyphae that contain septa are called septate hyphae.
Molds with hyphae that lack septa are called coenocytic hyphae .



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