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Culture media

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الكلية كلية التمريض     القسم قسم العلوم الطبية الاساسية     المرحلة 2
أستاذ المادة ميس هادي جبر       22/01/2016 12:01:13
Lab/3:-Culture media
The survival and growth of microorganisms depend on available nutrients and a favorable growth environment. These environments contain energy source, fundamental units & necessary contents for built and conformation the cell compartments, these fundamental units are the sources of carbon (C) & Nitrogen (N) (the materials that all the microorganisms that need them), and these culture media contain high percentage of H2O & other metals such as : Cu , Zn , metallic salts sometimes vitamins and gases such as O2 . The culture media differ depending on microbes needing to nutrition.
The media divided to three types depending on their contents
Natural media: contain natural materials such as meat extract & peptone. used for growth large numbers of microorganisms.
Synthetic media : organic & non organic chemicals inter in the its conformation.
semi synthetic media : contain nature & chemical materials most of culture media due to this type of culture media, while the media can be divided to three types depending on physical state:
Liquid culture media
Semi solid culture media
Solid culture media
Culture media packaging
Liquid culture media (broth)) :- The liquid culture media package before sterilization in test tubes , small flasks ,screw capped bottles
solid culture media (agar) :- The solid culture media package in the tubes for getting of deep agar slob before sterilization , while distribution of agar in the petri dishes after sterilization with (pour plate method).

Inoculation of Media:-
The word of culture is called of inoculation the microorganism in culture media There are two terms must be know : the( pure culture) : is the culture that have one species of microbes while the (mixed culture ) it contain two or more species of microorganisms.
Streak plate technique
Spreading methods
This method using with diluting series of culture and using ofL_shaped glass rod.
Pour plate Technique
The original sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies upon plating this method using for microorganisms count.
Stapping method
Note:-
The liquid media inoculating with loop while slant agar or slob agar in the tube can be inculcated with streaking on the surface of slob agar.


المادة المعروضة اعلاه هي مدخل الى المحاضرة المرفوعة بواسطة استاذ(ة) المادة . وقد تبدو لك غير متكاملة . حيث يضع استاذ المادة في بعض الاحيان فقط الجزء الاول من المحاضرة من اجل الاطلاع على ما ستقوم بتحميله لاحقا . في نظام التعليم الالكتروني نوفر هذه الخدمة لكي نبقيك على اطلاع حول محتوى الملف الذي ستقوم بتحميله .
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